Gene Construction Kit



Construction

:: DESCRIPTION

The construction of overproducing E. Coli strains is a difficult and time-consuming task, primarily because of two factors: (1) the requirement that the gene or complementary DNA to be expressed be refashioned from a molecularly monoclonal source, and (2) the requirement to precisely assemble specific DNA sequences during construction of the.

The Gene Construction Kit®(GCK) program has been the preferred plasmid mapping software of leading researchers for more than 20 years. GCK allows easy manipulation of DNA sequences, either graphically or as sequence text – quickly saving users both time and money. GCK eliminates tedious examination of DNA sequence data by automatically identifying open reading frames (ORF’s), keeping track of sticky ends during cutting and pasting of restriction enzyme digestion fragments, assisting with PCR primer design, and enabling comprehensive annotation of DNA sequence features. This DNA analysis software allows multiple files to be opened and displayed simultaneously, allowing DNA sequences to easily be copied and pasted between plasmids and vectors to represent real-world DNA cloning protocols. The Gene Construction Kit software is available for both Windows and Macintosh users, and files can be shared across platforms allowing for easy collaboration.

::DEVELOPER

:: SCREENSHOTS

:: REQUIREMENTS

  • Windows / MacOsX

The Universal GenomeWalker 2.0 Kit is a complete kit that contains all the reagents you need to construct gene libraries and perform PCR-based 'walking' in the genomic DNA of any species. In addition to the Universal GenomeWalker library construction components, the kit includes an Advantage 2 PCR Kit as well as the NucleoSpin Tissue. IDT cloning vectors. All of our synthetic gene products are provided in an optimized cloning vector that is ready to be transformed into E. Coli.To optimize manufacturing and delivery times, synthetic genes ≤5 kb in length are delivered in a “best-fit” vector (Table 1), for which you can choose between ampicillin or kanamycin selection markers. CAR-Manu™ Human T Cell Enrichment Kit (CAR-K-Z004) Add to Cart Datasheet. CAR-Manu™ PCR Purification Kit (CAR-K-Z003) Add to Cart Datasheet. CAR-Manu™ PCR Cleanup Kit (CAR-K-Z002) Add to Cart Datasheet. CAR-Manu™ PCR Amplification Kit (CAR-K-Z001) Add to Cart Datasheet. CellRapeutics™ In Fusion Cloning Kit (CART-017CL) Add to Cart.

:: DOWNLOAD

Gene Construction Kit Demo

Gene Construction Kit 4.5

:: MORE INFORMATION